UreB

基本解釋

Methods BALB/c mice and rabbits were immunized with rUreB.The specific antibodies to UreB were analyzed by agarose diffusion, ELISA and Western blot.
方法重組尿素酶B亞單位注射免疫BALB/c小鼠、家兔,雙向瓊脂擴散、ELISA、免疫印跡分析特異抗體的產(chǎn)生及性質(zhì)。
Recombinant pIRES ureB IL 2 and pIRES ureB were transfected into Cos 7 cells using LipofectAMINE TM 2000, the immunogenicity of UreB and IL 2 protein expressed was detected with SDS PAGE and Western blot.
通過脂質(zhì)體法將重組載體 pIRES ureB和pIRES ureB IL 2轉(zhuǎn)染Cos 7細胞 ,SDS PAGE及Westernblot法檢測表達蛋白的免疫性。
Construct rice expression vector of CTB-ureB fusion gene pCWUN:(1) Fusion and clone of CTB gene and ureB gene: CTB gene and Hp ureB gene were amplified by high-fidelity PCR respectively from plasmid pGEM-CTB and Hp genome, and then they were fused by PCR.
構(gòu)建CTB-ureB融合基因的水稻表達載體pCWUN：
Keywords Helicobacter pylori;DNA vaccine;UreB;HspA;
幽門螺桿菌;DNA疫苗;尿素酶;熱休克蛋白A;
Keywords antigen epitope;Urease B （UreB）;antigenicity;identification;screening;fluorescence polarization （FP）;
抗原表位;尿素酶B;抗原性;鑒定;篩選;熒光偏振;