affinity chromatography

基本解釋

Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Antibodies are purified by protein A and peptide affinity chromatography.
多克隆抗體通過(guò)用DYKDDDDK合成肽免疫動(dòng)物制備?？贵w用蛋白A和多肽親和色譜法純化。
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In the present study, recombinant human IRF-7 was expressed in Escherichia coli, purified by affinity chromatography, and utilized to raise polyclonal antibody in BALB/c mice.
本文在大腸桿菌中表達(dá)了重組IRF-7，利用親和層析的方法進(jìn)行了純化，并制備了鼠抗IRF-7的多克隆抗體。
The expressed protein was purified by one-step affinity chromatography, and its ability of binding plasmid was investigated via gel retardation experiments.
表達(dá)產(chǎn)物用親和層析一步法進(jìn)行純化，然后用凝膠阻滯試驗(yàn)觀察重組蛋白結(jié)合質(zhì)粒的能力。

親和層析
We isolated the LN receptor from the tissue of Lewis lung carcinoma by affinity chromatography.
本文采用親和層析法從小鼠Lewis肺癌組織中分離LN受體并對(duì)其理化性質(zhì)進(jìn)行研究。
親和色譜
親和純化
The His-ABPG fusion protein was obtained using the affinity chromatography and in a size of about 68.5 kDa.
利用表達(dá)質(zhì)粒上的His融合標(biāo)簽進(jìn)行親和純化,獲得電泳純?nèi)诤系鞍?其大小約為68.5 kDa。
親和色譜法

親和層析
The NS1 protein was acquired by the purification of chitin affinity chromatography.
經(jīng)幾丁質(zhì)柱親和層析純化得到NS1蛋白。
親和色譜
Methods We added perchloric acid into human plasma. Apolipoprotein H was separated and purified by ion exchange chromatography and affinity chromatography.
方法用高氯酸預(yù)處理血漿,以離子交換色譜和親和色譜相結(jié)合的方法進(jìn)行蛋白質(zhì)的分離和純化。
受體色譜