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Method 4 kinds of block buffer, the different concentration of anti-MIF and biotin-anti-MIF, the different dilution of streptavidin-HRP are used for titration of ELISA test. 方法采用方陣滴定法，比較4種封閉液、不同稀釋度的捕獲抗體、檢測抗體和辣根過氧化酶標(biāo)記鏈菌素對檢測結(jié)果的影響，確定ELISA法的最佳實驗條件。
The analytic results by DSC were verified by aniline titration analysis and FTIR analysis, which further indicated that it was available to block isocyanate by sodium bisulfate. 采用苯胺滴加的方法、FTIR的研究方法，驗證亞硫酸氫鈉封閉異氰酸酯的反應(yīng)的可行性，并進一步考證了用DSC方法得到的解封閉溫度的正確性。