gene-splicing
基本解釋
- n.基因剪切
英漢例句
- Compared to traditional crossbreeding, gene splicing takes about half as much time to develop a new crop or animal variety.
與傳統(tǒng)的雜交育種相比，基因剪接約需多半的時間開發(fā)一種新的作物或動物品種。 - Scientists have used gene splicing to develop modified crop plants, new drugs, pest-resistant plants, and animals that grow rapidly.
科學(xué)家們利用基因拼接開發(fā)改良作物，新的藥物，抗蟲植物和動物的迅速增長。 - Gene splicing 1.The joining of EXONS after the INTRON sequences have been removed, to produce functional messenger RNA that leaves the nucleus to undergo TRANSCRIPTION.
基因拼接：1.;是將內(nèi)含子序列剪切掉并使外顯子相連接的過程，所形成的功能性信使RNA繼續(xù)留在核中進一步轉(zhuǎn)錄。 - Methods:The full-length gene of NY-ESO-1 was generated by gene splicing method and the recombinant expression vector NY-ESO-1-pET-28a (+) was constructed. E. coli BL21 (DE3) bearing the plasmid was induced with IPTG for protein production.
方法:通過全基因拼接獲得NY-ESO-1基因,構(gòu)建重組表達載體NY-ESO-1-pET28a(+),在大腸桿菌BL21(DE3)中利用IPTG誘導(dǎo)獲得表達,利用單克隆抗體進行Western印跡鑒定,通過Ni柱親和純化獲得純化蛋白。 - Designed and synthesised 4 fragments of antibacterial peptide B gene, then these fragments were combinated into one expected fragment by the method of gene splicing by overlap extension (gene SOEing).
摘要人工設(shè)計并合成了抗菌肽B基因的4個寡聚核苷酸片段，通過重疊區(qū)擴增法，擴增出了相當(dāng)于抗菌肽基因全長的寡聚核苷酸片段。