over-lap pcr

The two tandem fragment of Aβ obtaining by overlap PCR technique was cloned into plant expression vector pBIDST(containing double 35S promoter and TEV enhancer).
本試驗利用重疊PCR技術(shù)獲得2Aβ串聯(lián)基因片段,并將其克隆到植物表達(dá)載體pBIDST(含雙35S啟動子和TEV增強子)中。