overlap-PCR

基本解釋

The H chain and L chain gene of anti_HBsAg fab were connected to construct single_chain fab gene using overlap PCR techniques.
采用重疊PCR技術(shù) ,以抗乙肝表面抗原 (HBsAg)IgG鉸鏈區(qū)基因?yàn)長(zhǎng)inker將Fab抗體基因的重鏈和輕鏈連接起來(lái) ,構(gòu)成單鏈Fab基因。
Methods: The gene fragment encoding the Z domain of Protein A(Protein A-Z) was generated by Overlap PCR amplification, and was cloned into TA-cloning vector pMD-18T.
方法:應(yīng)用Overlap PCR的方法制備Protein A的Z結(jié)構(gòu)域(Protein A-Z),克隆于pMD-18T載體構(gòu)建PA-Z/pMD-18T重組質(zhì)粒。
By designing four primers for an overlapping PCR, we created a fusion gene Ddl cat encoding human TNF receptor I death domain and chloramphenicol acetyltransferase(CAT).
通過(guò)設(shè)計(jì)4個(gè)引物進(jìn)行重疊（overlapping）PCR，由此克隆了人腫瘤壞死因子受體I死亡域（deathdomain）與氯霉素乙酰轉(zhuǎn)移酶（CAT）的融合蛋白基因（DdLcat）。
Keywords Lagurus lagurus;zona pellucida 3;overlap PCR;codon optimization;prokaryotic expression;
草原兔尾鼠;卵透明帶3;重疊PCR;密碼子優(yōu)化;原核表達(dá);
There is no question of overlap between the two courses.
這兩門課程之間不存在重疊的問(wèn)題。