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    primer-design

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    • ITS sequences of ten kinds of Iris plants and an outgroup were obtained by primer design, PCR, gene cloning, sequencing and cluster analysis.
      通過引物設(shè)計(jì)、PCR、基因克隆、測序,獲得了10種鳶尾屬植物和1個(gè)外類群種的ITS序列。
    • The primer design is a key step for RAMP and REMAP, in which anchored simple sequence repeat primers were used.
      摘要引物設(shè)計(jì)是RAMP和REMAP標(biāo)記分析的關(guān)鍵,二者都使用錨定的微衛(wèi)星序列引物。
    • In China these viruses share high homology with Takahaski’s strains which might be good standards for primer designation.
      因而,設(shè)計(jì)pcr引物時(shí)宜選擇Takahashi等分離株為標(biāo)準(zhǔn)。
    • In this study ,the PCR technique was developed for serotyping A.pleuropneumoniae using a set of specific primer designated for the apxI,apxII,apxIII and apxIV genes.
      APP 12種血清型分別具有不同的毒素基因(apxI,apxII,apxIII,apxIV)。
    • An "agg" codon between 1039-1041bp in PLF gene was replaced with a "taa" stop codon through primers design and PCR.
      豬乳鐵蛋白基因1039-1041bp處為agg密碼子,在設(shè)計(jì)引物時(shí)突變?yōu)閠aa終止密碼子。
    • Using the primer designed from the gene of dihydroflavonol 4-reductase (DFR) in Arabidopsis thaliana, using the genomic DNA of Sichuan yellow Ziyejie as template, we obtained the DFR gene fragment.
      參照在擬南芥4-二氫黃酮還原酶基因序列,設(shè)計(jì)基因?qū)R灰?,靶向擴(kuò)增克隆目的基因片段。
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