ECOR

常見例句

SV40T and FMDV-2A were ligated and cloned to the sites , sal I and EcoR I of pBGTKlox to make pBTGTKlox.
連接SV40T與FMDV-2A,回收后用EcoR I和Sal I酶切連接至pBGTKlox載體的相應(yīng)位點(diǎn),構(gòu)成的新載體稱為pBTGTKlox。
P16-promoter-luc-pGL2-Basic plasmid DNA was prepared after plasmid amplification, and digested by EcoR I and Hind III.
擴(kuò)增p 16 promoter一luc一pGLZ一Basic報告基因表達(dá)質(zhì)粒并提取其DNA。
Then the pEGF-6-his-tag fragment was cloned into plasmid pPIC9 at the sites of Xho I and EcoR I.
然后將PCR產(chǎn)物克隆到分泌表達(dá)載體pPIC9構(gòu)建了pPIC9-pEGF。
The circle plasmid-like DNA pC1 was cloned into the EcoR site of pUC19 using E.coli JM109 as host.
將環(huán)形類質(zhì)粒pC1連到pUC19的EcoR位點(diǎn)上，克隆至E.;coli JM109中。
The obtained bands were cloned into pGEM T-Easy vector, and the positive clone were confirmed by EcoR I enzyme digestion.
將所獲片段克隆入pGEN T-Easy載體，EcoR I酶切鑒定。
In addition, the amplified IL-15 cDNA fragment from plasmid pBS-IL15 was digested with BamH I and cloned into EcoR I (filled in)/BamH I pBV220 vector.
另外，將經(jīng)BamHI酶切的IL-15cDNA擴(kuò)增片斷與經(jīng)EcoRI酶切、補(bǔ)平、再經(jīng)BamHI酶切的pBV220載體相連，構(gòu)建成重組質(zhì)粒pBVB-IL15。