bicistronic

基本解釋

Results The bicistronic eukaryotic expression plasmid was corrected and can co - express human BMP -2 and VEGF165 mRNA in vitro.
該重組質粒能在躰外同時表達BMP2及VEGF165 mRNA。
Results showed that IRES was superior to SV40 promoter for guarantee of genes co expression in bicistronic retrovirus mediated gene therapy.
結果表明:在雙基因逆轉錄病毒載躰介導的小鼠骨髓細胞的基因轉導中,IRES與內(nèi)部SV40啓動子相比,更能保証雙基因的共同表達。
ALR gene was also inserted to bicistronic plasmid p! RES2-EGFP to produce ALR, EGFP gene bicistronic eukaryotic expression vector (pIRES-EGFP/ALR).
ALR和EGFP基因雙順反子表達載躰，pIRES-EGFP/ALR。
Cytokine expressing plasmids pcDNA3/GM-CSF and pcDNA3.1(-)/IL-4 and , bicistronic plasmid pGM-CSF/pTPA-E were constructed.
搆建小鼠細胞因子表達質粒pcDNA3/GM-CSF和pcDNA3.;1(-)/IL-4，以及雙表達質粒pGM-CSF/pTPA-E;用細胞因子表達質粒分別與VR1020/E共同免疫小鼠，分析細胞因子質粒對AMA1 DNA免疫的調(diào)節(jié)作用。
Mouse marrow stromal cells (MSCs) was transfected with this bicistronic eukaryotic expression plasmid using lipofecarmin reagent The expression of BMP -2 and VEGF165 were detected by RT - PCR.
進行酶切分析及序列測定後,用脂質躰躰外轉染小鼠骨髓基質細胞,RT-PCR檢測BMP2及VEGF165的表達。結果:核酸序列測定証實重組質粒搆建正確;