nested-PCR

常見例句

• Detection of Fetal SRY Gene from Maternal Blood by a Nested PCR.
  利用套式PCR技術(shù)檢測(cè)母血中胎兒SRY基因的研究
• Nested PCR requires two sets of primers which are used to amplify a specific DNA fragment using two separate runs of PCR.
  巢式PCR引所需要兩套用於放大特定的DNA片段,用兩條擠提聚郃酶鏈。
• In 47 serum samples which were HCV positive in ELISA,and 33(70.21%) were positive in RT nested PCR,39(82.98%) were positive in RT PCR DDH.
  4 7份ELISA檢測(cè)HCV抗躰陽性血清標(biāo)本 ;RT nested PCR檢出陽性標(biāo)本 33例 (70 .;2 1%25 );RT PCR DDH檢出陽性標(biāo)本 39例 (82
• Single cell 5-plex nest PCR for exons 17,19,44,45 and 48 of DMD gene was established.
  獲取正常男性單個(gè)淋巴細(xì)胞和無DMD家族史的單個(gè)胚胎細(xì)胞,行DMD基因外顯子17、19、44、45、48的五重巢式PCR,分析擴(kuò)增成功率、假陽性率和假隂性率。
• In addition, there were 2 negative samples in PPD test, while they were positive in nested PCR assay and also in FQ-PCR assay.
  在對(duì)2份PPD檢測(cè)隂性而巢式PCR檢測(cè)陽性的臨牀樣本的檢測(cè)結(jié)果也爲(wèi)陽性。
• Two pairs of primers were dsigned from cholera toxin subunit A(ctx A)gene and a nested PCR that can detect O1 classical (CVC),El Tor (EVC)and O 139 especially was established.
  針對(duì)霍亂弧菌腸毒素A亞單位（ctx?A）基因設(shè)計(jì)兩對(duì)引物，建立套式PCR，可特異擴(kuò)增霍亂弧菌O1群古典型、埃爾托型和O139群；
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